Fluorescence lim yog cov khoom siv tseem ceeb siv hauv biomedical thiab life science instruments.Nws lub luag haujlwm tseem ceeb yog cais thiab xaiv cov yam ntxwv wavelength spectrum los ntawm lub teeb pom kev zoo thiab emission fluorescence ntawm cov khoom hauv biomedical fluorescence tshuaj xyuas thiab tshuaj xyuas qhov system.Fluorescence filters feem ntau yog tus cwj pwm los ntawm kev txiav tawm qhov tob thiab qis autofluorescence.Feem ntau, ntau cov ntxaij lim dej tuaj yeem ua ke ua ke los tsim cov lim dej fluorescence, uas muaj kev ua haujlwm siab.
Fluorescence filter yog siv nyob rau hauv real-time fluorescence quantitative PCR ntsuas.uas yog dav siv nyob rau hauv molecular biology thiab zaub mov kev nyab xeeb nrhiav kom tau thiab pej xeem noj qab haus huv kev sib kis mob thiab lwm yam lag luam.
Fluorescence lim yog siv los kuaj xyuas ntau yam dyes thiab sojntsuam, piv txwv li:
FAM / SYBR Ntsuab / Ntsuab / HEX / TET / Cy3 / JOE / ROX / Cy3.5 / Texas Liab, Cy5 / LC Red640, Cy5.5 thiab lwm yam
Txheej txheem | (IAD Hard Txheej) | ||
Lub wavelength | Ex (nm) | Em (nm) | Hla |
| 470-30 : kuv | 525-20 : kuv | > 6 |
| 523-20 : kuv | 564-20 : kuv | > 6 |
| 543-20 : kuv | 584-20 : kuv | > 6 |
| 571-20 : kuv | 612-20 : kuv | > 6 |
| 628-35 : kuv | 692-45 : kuv | > 6 |
Thaiv | OD> 6 @ 200 ~ 900nm lossis @ 200 ~ 1200nm | ||
Txoj kab nqes (nm) | 50% ~ OD5 <10nm | ||
Hla | OD> 6 | ||
Loj (mm) | Φ4mm, Φ12mm, Φ12.7mm, Φ25.4mm thiab lwm yam |